ERR1142448_2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1142448_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	77069
Sequences flagged as poor quality	0
Sequence length	125
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	434	0.5631317390909445	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	331	0.42948526644954516	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	235	0.30492156379348373	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	204	0.26469786814413054	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	174	0.22577171106411137	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	170	0.2205815567867755	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	169	0.2192840182174415	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	163	0.21149878680143766	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	160	0.20760617109343577	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	142	0.18425047684542423	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	142	0.18425047684542423	No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	140	0.1816553997067563	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	139	0.1803578611374223	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	138	0.17906032256808835	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	135	0.17516770686008643	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	116	0.15051447404274093	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	106	0.13753908834940118	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	105	0.1362415497800672	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	101	0.13105139550273132	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	98	0.1271587797947294	No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT	97	0.1258612412253954	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	94	0.12196862551739351	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	92	0.11937354837872555	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	91	0.11807600980939159	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	90	0.11677847124005761	No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA	87	0.11288585553205568	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	87	0.11288585553205568	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	85	0.11029077839338775	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	84	0.10899323982405376	No Hit
GTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAA	84	0.10899323982405376	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	82	0.10639816268538582	No Hit
TATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAA	79	0.1025055469773839	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	79	0.1025055469773839	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	79	0.1025055469773839	No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA	78	0.10120800840804993	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATA	30	4.1878397E-5	79.52711	1
TCGGGTA	25	0.0020350309	71.52768	4
GTACAAG	30	0.0041808975	59.64533	1
TACATAA	55	1.2854713E-5	54.187634	2
GTAAAAA	80	3.7118298E-8	52.189667	1
AGTACAA	35	0.007713993	51.091194	2
GAACAAA	60	2.1429336E-5	49.70444	1
ACTGTGC	65	3.4660377E-5	45.821167	8
TACTGTG	75	8.063221E-5	39.711678	7
CTAGAAG	65	0.0019108048	36.68086	3
ATTCGAG	35	0.0027749324	34.016388	26-27
CTGTGCG	75	0.0038417622	31.810844	9
ACCATAA	75	0.0038540787	31.79008	2
GAGAACA	50	4.358165E-4	29.822666	12-13
TAGTACT	80	0.005285031	29.803196	4
TGGGGGG	120	3.639733E-5	29.764343	6
GTACTGT	80	0.00531889	29.764341	6
GACTGCT	40	0.0053353207	29.764341	6
GCAAGTT	40	0.0053523555	29.744951	78-79
CGCAAGT	40	0.0053523555	29.744951	76-77

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content