ERR1142448_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1142448_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	77069
Sequences flagged as poor quality	0
Sequence length	125
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	861	1.1171807081965512	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	787	1.021162854065837	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	533	0.6915880574550078	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	399	0.5177178891642554	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	331	0.42948526644954516	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	327	0.42429511217220933	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	325	0.42170003503354136	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	304	0.39445172507752796	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	276	0.3581206451361767	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	200	0.25950771386679466	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	172	0.22317663392544346	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	169	0.2192840182174415	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	163	0.21149878680143766	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	161	0.20890370966276972	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	149	0.19333324683076203	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	148	0.19203570826142807	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	140	0.1816553997067563	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	128	0.1660849368747486	No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	121	0.1570021668894108	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	120	0.1557046283200768	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	119	0.15440708975074285	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	114	0.14791939690407296	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	112	0.14532431976540502	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	109	0.1414317040574031	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	103	0.13364647264139926	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	101	0.13105139550273132	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	100	0.12975385693339733	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT	98	0.1271587797947294	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	96	0.12456370265606145	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	95	0.12326616408672747	No Hit
GTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAA	93	0.12067108694805953	No Hit
GGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGA	93	0.12067108694805953	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	91	0.11807600980939159	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	89	0.11548093267072362	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	88	0.11418339410138967	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	88	0.11418339410138967	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	88	0.11418339410138967	No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG	83	0.1076957012547198	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	83	0.1076957012547198	No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT	80	0.10380308554671788	No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA	80	0.10380308554671788	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	80	0.10380308554671788	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCAGA	30	2.5338522E-9	119.0526	1
GCGTAAG	15	2.6873004E-4	119.0526	1
GTCAACG	30	3.5370977E-7	99.2105	1
CCTGTAG	15	0.004211352	59.56498	80-81
GGTTTCA	30	0.004214196	59.5263	1
CAGTGTA	30	0.004225053	59.48767	2
ACTAAGC	30	0.004225053	59.48767	8
GCCTAGC	15	0.004233076	59.48767	42-43
AGCCTAA	15	0.004233076	59.48767	46-47
GGCCTGC	15	0.004233076	59.48767	108-109
GTGCGAT	15	0.004233076	59.48767	100-101
AGTGTAC	30	0.004225053	59.48767	3
TGAATCG	35	0.007775205	50.989433	5
ACTCGTA	25	5.3235877E-4	47.590137	114-115
ACGCCAC	30	0.0013063215	39.658447	40-41
GCACGCC	30	0.0013063215	39.658447	38-39
CGTCATA	30	0.0013063215	39.658447	50-51
AAGTCAC	30	0.0013063215	39.658447	96-97
GCGCACG	40	1.1941156E-4	37.179794	36-37
TGCGAGG	35	0.0027578722	34.05926	84-85

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content