##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142422_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2383
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.171212757028954	27.0	14.0	33.0	14.0	33.0
2	22.33822912295426	27.0	14.0	33.0	14.0	33.0
3	22.62778010910617	27.0	14.0	33.0	14.0	33.0
4	22.161980696600924	14.0	14.0	33.0	14.0	33.0
5	22.64498531263114	27.0	14.0	33.0	14.0	33.0
6	23.871590432228285	27.0	14.0	33.0	14.0	37.0
7	24.224926563155687	27.0	14.0	37.0	14.0	37.0
8	23.709190096516995	27.0	14.0	33.0	14.0	37.0
9	23.530004196391104	27.0	14.0	33.0	14.0	37.0
10-11	23.612673101133026	27.0	14.0	33.0	14.0	37.0
12-13	23.758497691984893	27.0	14.0	33.0	14.0	37.0
14-15	23.514477549307596	27.0	14.0	33.0	14.0	37.0
16-17	23.483424255140577	27.0	14.0	33.0	14.0	37.0
18-19	23.269198489299203	24.5	14.0	33.0	14.0	37.0
20-21	23.273814519513216	24.5	14.0	33.0	14.0	37.0
22-23	23.15589592950063	24.5	14.0	33.0	14.0	37.0
24-25	23.059588753671843	22.0	14.0	33.0	14.0	37.0
26-27	22.998741082668904	18.0	14.0	33.0	14.0	37.0
28-29	22.79122954259337	14.0	14.0	33.0	14.0	37.0
30-31	22.748845992446498	18.0	14.0	33.0	14.0	37.0
32-33	22.984053713806126	18.0	14.0	33.0	14.0	37.0
34-35	22.65526647083508	14.0	14.0	33.0	14.0	37.0
36-37	22.781997482165337	18.0	14.0	33.0	14.0	37.0
38-39	22.866974402014268	18.0	14.0	33.0	14.0	37.0
40-41	22.5889634913974	14.0	14.0	33.0	14.0	37.0
42-43	22.661141418380193	14.0	14.0	33.0	14.0	37.0
44-45	22.654007553503988	14.0	14.0	33.0	14.0	37.0
46-47	22.74968527066723	18.0	14.0	33.0	14.0	37.0
48-49	22.78031892572388	14.0	14.0	33.0	14.0	37.0
50-51	22.626101552664707	14.0	14.0	33.0	14.0	37.0
52-53	22.87117079311792	22.0	14.0	33.0	14.0	37.0
54-55	22.63491397398238	18.0	14.0	33.0	14.0	37.0
56-57	22.679815358791437	14.0	14.0	33.0	14.0	37.0
58-59	22.563155686109944	14.0	14.0	33.0	14.0	37.0
60-61	22.5744859420898	14.0	14.0	33.0	14.0	37.0
62-63	22.518464120856066	14.0	14.0	33.0	14.0	37.0
64-65	22.357532522031054	14.0	14.0	33.0	14.0	37.0
66-67	22.414393621485523	14.0	14.0	33.0	14.0	37.0
68-69	22.429290809903485	14.0	14.0	33.0	14.0	37.0
70-71	22.488250104909778	14.0	14.0	33.0	14.0	37.0
72-73	22.515946286193874	14.0	14.0	33.0	14.0	37.0
74-75	22.353126311372222	14.0	14.0	30.0	14.0	37.0
76-77	22.101972303818716	14.0	14.0	30.0	14.0	37.0
78-79	22.064204783885856	14.0	14.0	30.0	14.0	37.0
80-81	22.198908938313053	14.0	14.0	30.0	14.0	37.0
82-83	21.981955518254303	14.0	14.0	30.0	14.0	37.0
84-85	21.89131347041544	14.0	14.0	27.0	14.0	37.0
86-87	21.882291229542595	14.0	14.0	27.0	14.0	37.0
88-89	21.794796475031475	14.0	14.0	27.0	14.0	37.0
90-91	21.638900545530845	14.0	14.0	27.0	14.0	37.0
92-93	21.77087704574066	14.0	14.0	27.0	14.0	37.0
94-95	21.49496433067562	14.0	14.0	27.0	14.0	37.0
96-97	21.549307595467898	14.0	14.0	27.0	14.0	37.0
98-99	21.278220730172052	14.0	14.0	27.0	14.0	37.0
100-101	21.096726814939153	14.0	14.0	27.0	14.0	37.0
102-103	21.369911875786823	14.0	14.0	27.0	14.0	37.0
104-105	21.13365505665128	14.0	14.0	27.0	14.0	37.0
106-107	21.26961812840957	14.0	14.0	27.0	14.0	37.0
108-109	20.799202685690304	14.0	14.0	27.0	14.0	37.0
110-111	20.892992026856902	14.0	14.0	27.0	14.0	37.0
112-113	20.82522031053294	14.0	14.0	27.0	14.0	37.0
114-115	20.849769198489298	14.0	14.0	27.0	14.0	37.0
116-117	20.45279060008393	14.0	14.0	27.0	14.0	37.0
118-119	20.474821653378093	14.0	14.0	27.0	14.0	37.0
120-121	20.310742761225345	14.0	14.0	24.5	14.0	37.0
122-123	20.225765841376415	14.0	14.0	24.5	14.0	37.0
124-125	19.856903063365507	14.0	14.0	24.5	14.0	35.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
0	6.0
1	3.0
2	0.0
3	0.0
4	0.0
5	1.0
6	0.0
7	0.0
8	0.0
9	1.0
10	0.0
11	1.0
12	0.0
13	0.0
14	5.0
15	86.0
16	241.0
17	332.0
18	300.0
19	223.0
20	172.0
21	134.0
22	103.0
23	83.0
24	86.0
25	52.0
26	49.0
27	49.0
28	45.0
29	35.0
30	45.0
31	48.0
32	37.0
33	50.0
34	49.0
35	75.0
36	72.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.48838191803971	27.207435572454585	16.518800168990282	23.785382340515422
2	18.120522545301306	27.18078381795196	29.835651074589126	24.863042562157606
3	18.752633796881586	30.172777075431945	26.295828065739567	24.778761061946902
4	17.277707543194268	27.43362831858407	27.644332069110828	27.644332069110828
5	17.06700379266751	35.27180783817952	27.47576906868942	20.185419300463547
6	25.841750841750844	35.60606060606061	18.68686868686869	19.865319865319865
7	24.410774410774412	33.92255892255892	21.254208754208754	20.412457912457914
8	22.685185185185187	35.69023569023569	19.654882154882156	21.96969696969697
9	23.14814814814815	27.02020202020202	19.444444444444446	30.387205387205384
10-11	22.874579124579125	30.955387205387208	22.874579124579125	23.295454545454543
12-13	23.085016835016837	30.576599326599325	23.063973063973066	23.274410774410775
14-15	21.84732180514551	30.21931674398988	21.699704765921553	26.233656684943064
16-17	20.97931616715914	31.785563528915155	22.140143520472773	25.094976783452932
18-19	21.443647108484594	33.030814689742506	22.013507809202196	23.512030392570704
20-21	22.794428028704093	29.991557619248628	23.08991135500211	24.124102997045167
22-23	23.3642887294217	29.674968341072184	23.617560151962856	23.343182777543266
24-25	23.08991135500211	29.632756437315322	23.02659349936682	24.250738708315744
26-27	21.308016877637133	30.759493670886073	23.122362869198312	24.81012658227848
28-29	23.02659349936682	28.99957788096243	23.828619670747152	24.145208948923596
30-31	22.51054852320675	28.77637130801688	23.924050632911392	24.78902953586498
32-33	22.552742616033754	29.09282700421941	23.58649789029536	24.76793248945148
34-35	21.851929972579626	27.98987555368066	23.62370807846446	26.53448639527526
36-37	22.016026992830028	29.24926191480388	23.175875158161112	25.558835934204975
38-39	22.247996625896246	27.64656263180093	22.50105440742303	27.6043863348798
40-41	22.8806410797132	28.384647827920706	22.901729228173767	25.832981864192323
42-43	22.479966258962463	28.764234500210883	22.690847743568117	26.064951497258544
44-45	22.92281737663433	27.752003374103758	23.913960354280896	25.41121889498102
46-47	22.697576396206536	28.74604847207587	22.592202318229717	25.964172813487885
48-49	22.960151802656547	28.779253636938645	23.065570314147163	25.195024246257642
50-51	22.386172006745362	28.520236087689714	22.828836424957842	26.264755480607082
52-53	22.777075431942688	27.74968394437421	23.261694058154237	26.211546565528863
54-55	22.528977871443626	27.734457323498418	22.80295047418335	26.933614330874605
56-57	22.280775716694773	28.393760539629003	21.838111298482293	27.48735244519393
58-59	23.076923076923077	28.80927291886196	22.444678609062173	25.669125395152793
60-61	21.91780821917808	28.36670179135933	23.519494204425712	26.195995785036878
62-63	22.650653181626634	29.056047197640115	22.166034555415088	26.127265065318163
64-65	22.84028655710072	27.328276443320693	23.093131057732826	26.738305941845763
66-67	23.0185497470489	27.25548060708263	22.133220910623947	27.59274873524452
68-69	23.229342327150086	28.414839797639125	22.976391231028668	25.379426644182125
70-71	23.43519494204426	27.96628029504742	23.519494204425712	25.07903055848261
72-73	23.086654016445287	27.49314779675311	22.896900695762177	26.523297491039425
74-75	22.929399367755533	28.13487881981033	23.308746048472077	25.62697576396207
76-77	23.414120126448893	27.249736564805062	23.055848261327714	26.280295047418335
78-79	23.524451939291737	27.74030354131535	22.048903878583474	26.68634064080944
80-81	23.013698630136986	28.13487881981033	22.486828240252898	26.364594309799788
82-83	24.198988195615513	27.951096121416523	22.280775716694773	25.569139966273184
84-85	23.908918406072104	29.47501581277672	21.526460046384145	25.089605734767023
86-87	23.38811630847029	28.33965444584914	22.166034555415088	26.10619469026549
88-89	21.89673340358272	28.661749209694413	22.697576396206536	26.743940990516336
90-91	22.423603793466807	29.041095890410958	21.622760800842993	26.912539515279242
92-93	22.060682680151707	29.51959544879899	23.072060682680153	25.347661188369152
94-95	22.187104930467765	28.255372945638435	23.34597555836494	26.211546565528863
96-97	22.292456805731142	29.793510324483773	22.03961230509903	25.87442056468605
98-99	22.14029913629661	29.997893406361914	20.98167263534864	26.880134821992836
100-101	21.529386981251317	30.629871497788073	21.8453760269644	25.995365493996207
102-103	22.566371681415927	29.56173619890434	22.798145806995365	25.073746312684364
104-105	21.78676780446692	30.299199325748	22.14496418036241	25.76906868942267
106-107	22.78854254422915	30.623420387531592	21.545914069081718	25.04212299915754
108-109	21.192331999157364	31.135453970929007	21.487255108489574	26.184958921424055
110-111	22.966708807416772	30.23598820058997	21.00716392751791	25.790139064475348
112-113	21.8453760269644	31.177585843690753	21.150200126395617	25.82683800294923
114-115	22.367312552653747	31.065711878685764	20.07160909856782	26.49536647009267
116-117	20.98167263534864	31.28291552559511	21.95070570886876	25.784706130187484
118-119	21.798652064026957	31.42375737152485	21.040438079191237	25.73715248525695
120-121	22.40943555181129	31.381634372367312	20.871946082561077	25.33698399326032
122-123	21.019376579612466	31.71861836562763	21.75652906486942	25.50547598989048
124-125	20.65263157894737	32.484210526315785	21.6	25.263157894736842
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10.0
1	7.0
2	2.0
3	0.5
4	1.0
5	2.5
6	3.0
7	1.0
8	1.5
9	1.5
10	0.5
11	1.0
12	2.5
13	2.0
14	2.0
15	3.0
16	4.0
17	4.5
18	3.5
19	4.5
20	5.0
21	5.5
22	7.0
23	8.0
24	9.5
25	9.5
26	9.0
27	11.0
28	14.5
29	18.0
30	15.0
31	16.0
32	23.5
33	27.0
34	26.0
35	30.0
36	38.5
37	45.5
38	43.5
39	47.0
40	61.0
41	60.5
42	54.0
43	57.5
44	66.5
45	73.5
46	82.0
47	90.5
48	93.5
49	87.5
50	86.0
51	92.5
52	92.5
53	89.0
54	95.5
55	92.5
56	77.5
57	72.5
58	72.5
59	64.0
60	53.5
61	49.0
62	44.0
63	38.0
64	31.5
65	27.5
66	18.5
67	13.0
68	14.5
69	15.0
70	13.5
71	9.0
72	7.5
73	8.0
74	5.0
75	2.0
76	2.5
77	2.5
78	2.0
79	2.0
80	1.0
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.6714225765841376
2	0.41963911036508605
3	0.41963911036508605
4	0.41963911036508605
5	0.41963911036508605
6	0.2937473772555602
7	0.2937473772555602
8	0.2937473772555602
9	0.2937473772555602
10-11	0.2937473772555602
12-13	0.2937473772555602
14-15	0.5035669324381032
16-17	0.5874947545111204
18-19	0.5874947545111204
20-21	0.5874947545111204
22-23	0.5874947545111204
24-25	0.5874947545111204
26-27	0.5455308434746118
28-29	0.5874947545111204
30-31	0.5455308434746118
32-33	0.5455308434746118
34-35	0.5245488879563576
36-37	0.5035669324381032
38-39	0.5035669324381032
40-41	0.5035669324381032
42-43	0.5035669324381032
44-45	0.5035669324381032
46-47	0.4406210658833403
48-49	0.48258497691984886
50-51	0.46160302140159465
52-53	0.41963911036508605
54-55	0.4406210658833403
56-57	0.46160302140159465
58-59	0.4406210658833403
60-61	0.4406210658833403
62-63	0.41963911036508605
64-65	0.41963911036508605
66-67	0.46160302140159465
68-69	0.46160302140159465
70-71	0.4406210658833403
72-73	0.48258497691984886
74-75	0.4406210658833403
76-77	0.4406210658833403
78-79	0.46160302140159465
80-81	0.4406210658833403
82-83	0.46160302140159465
84-85	0.48258497691984886
86-87	0.41963911036508605
88-89	0.4406210658833403
90-91	0.4406210658833403
92-93	0.41963911036508605
94-95	0.41963911036508605
96-97	0.41963911036508605
98-99	0.39865715484683173
100-101	0.39865715484683173
102-103	0.41963911036508605
104-105	0.41963911036508605
106-107	0.37767519932857746
108-109	0.39865715484683173
110-111	0.41963911036508605
112-113	0.39865715484683173
114-115	0.37767519932857746
116-117	0.39865715484683173
118-119	0.37767519932857746
120-121	0.37767519932857746
122-123	0.37767519932857746
124-125	0.3357112882920688
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2383.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.11875786823332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.70364098221846	98.82501049097776
2	0.12701100762066045	0.2517834662190516
3	0.0846740050804403	0.2517834662190516
4	0.0	0.0
5	0.0	0.0
6	0.04233700254022015	0.2517834662190516
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.04233700254022015	0.41963911036508605
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10	0.41963911036508605	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	6	0.2517834662190516	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.1258917331095258	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	3	0.1258917331095258	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0209819555182543	0.0
28-29	0.0	0.0	0.0	0.0419639110365086	0.0
30-31	0.0	0.0	0.0	0.0419639110365086	0.0
32-33	0.0	0.0	0.0	0.10490977759127151	0.0
34-35	0.0	0.0	0.0	0.1258917331095258	0.0
36-37	0.0	0.0	0.0	0.1888375996642887	0.0
38-39	0.0	0.0	0.0	0.2727654217373059	0.0
40-41	0.0	0.0	0.0	0.2937473772555602	0.0
42-43	0.0	0.0	0.0	0.31472933277381454	0.0
44-45	0.0	0.0	0.0	0.3566932438103231	0.0
46-47	0.0	0.0	0.0	0.4825849769198489	0.0
48-49	0.0	0.0	0.0	0.5035669324381032	0.0
50-51	0.0	0.0	0.0	0.5665127989928661	0.0
52-53	0.0	0.0	0.0	0.5874947545111204	0.0
54-55	0.0	0.0	0.0	0.6294586655476291	0.0
56-57	0.0	0.0	0.0	0.6294586655476291	0.0
58-59	0.0	0.0	0.0	0.692404532102392	0.0
60-61	0.0	0.0	0.0	0.8182962652119177	0.0
62-63	0.0	0.0	0.0	0.8812421317666806	0.0
64-65	0.0	0.0	0.0	0.9441879983214436	0.0
66-67	0.0	0.0	0.0	0.9651699538396978	0.0
68-69	0.0	0.0	0.0	0.9651699538396978	0.0
70-71	0.0	0.0	0.0	1.028115820394461	0.0
72-73	0.0	0.0	0.0	1.0700797314309693	0.0
74-75	0.0	0.0	0.0	1.195971464540495	0.0
76-77	0.0	0.0	0.0	1.3008812421317666	0.0
78-79	0.0	0.0	0.0	1.4687368862778012	0.0
80-81	0.0	0.0	0.0	1.594628619387327	0.0
82-83	0.0	0.0	0.0	1.6995383969785984	0.0
84-85	0.0	0.0	0.0	1.80444817456987	0.0
86-87	0.0	0.0	0.0	1.8883759966428872	0.0
88-89	0.0	0.0	0.0	1.9723038187159043	0.0
90-91	0.0	0.0	0.0	2.0981955518254303	0.0
92-93	0.0	0.0	0.0	2.2240872849349556	0.0
94-95	0.0	0.0	0.0	2.2660511959714644	0.0
96-97	0.0	0.0	0.0	2.3499790180444817	0.0
98-99	0.0	0.0	0.0	2.4339068401174986	0.0
100-101	0.0	0.0	0.0	2.580780528745279	0.0
102-103	0.0	0.0	0.0	2.643726395300042	0.0
104-105	0.0	0.0	0.0	2.6647083508182963	0.0
106-107	0.0	0.0	0.0	2.790600083927822	0.0
108-109	0.0	0.0	0.0	2.958455728073856	0.0
110-111	0.0	0.0	0.0	3.1263113722198907	0.0
112-113	0.0	0.0	0.0	3.2731850608476707	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE