##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142422_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2383
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.091061686949224	27.0	14.0	33.0	14.0	33.0
2	26.583718002517834	27.0	27.0	33.0	14.0	33.0
3	28.512798992866134	33.0	27.0	33.0	14.0	33.0
4	29.988669744020143	33.0	27.0	33.0	27.0	33.0
5	30.56021821233739	33.0	33.0	33.0	27.0	33.0
6	33.308854385228706	37.0	33.0	37.0	27.0	37.0
7	33.59546789760806	37.0	33.0	37.0	27.0	37.0
8	33.97608057070919	37.0	33.0	37.0	27.0	37.0
9	34.15316827528326	37.0	33.0	37.0	27.0	37.0
10-11	34.321023919429294	37.0	35.0	37.0	27.0	37.0
12-13	34.54825849769199	37.0	37.0	37.0	27.0	37.0
14-15	34.461603021401594	37.0	37.0	37.0	27.0	37.0
16-17	34.387536718422155	37.0	37.0	37.0	27.0	37.0
18-19	34.37221989089383	37.0	37.0	37.0	27.0	37.0
20-21	34.266051195971464	37.0	37.0	37.0	27.0	37.0
22-23	34.23667645824591	37.0	33.0	37.0	27.0	37.0
24-25	34.12798992866135	37.0	33.0	37.0	27.0	37.0
26-27	33.74150230801511	37.0	33.0	37.0	27.0	37.0
28-29	33.45153168275283	37.0	33.0	37.0	20.5	37.0
30-31	33.37096097356273	37.0	33.0	37.0	14.0	37.0
32-33	33.361309274024336	37.0	33.0	37.0	14.0	37.0
34-35	33.22282836760386	37.0	33.0	37.0	14.0	37.0
36-37	33.125891733109526	37.0	33.0	37.0	14.0	37.0
38-39	33.36508602601762	37.0	33.0	37.0	18.0	37.0
40-41	33.07889215274864	37.0	33.0	37.0	14.0	37.0
42-43	32.77087704574066	37.0	33.0	37.0	14.0	37.0
44-45	32.64750314729333	37.0	33.0	37.0	14.0	37.0
46-47	32.70939991607218	37.0	33.0	37.0	14.0	37.0
48-49	32.56336550566513	37.0	33.0	37.0	14.0	37.0
50-51	32.47083508182963	37.0	33.0	37.0	14.0	37.0
52-53	32.48489299202686	37.0	33.0	37.0	14.0	37.0
54-55	32.16869492236677	37.0	30.0	37.0	14.0	37.0
56-57	32.20981955518255	37.0	33.0	37.0	14.0	37.0
58-59	32.28073856483424	37.0	33.0	37.0	14.0	37.0
60-61	31.965589592950064	37.0	30.0	37.0	14.0	37.0
62-63	31.915652538816616	37.0	27.0	37.0	14.0	37.0
64-65	32.04175409148132	37.0	27.0	37.0	14.0	37.0
66-67	31.896558959295007	37.0	27.0	37.0	14.0	37.0
68-69	31.826689047419222	37.0	27.0	37.0	14.0	37.0
70-71	31.78304657994125	37.0	27.0	37.0	14.0	37.0
72-73	31.881451951321864	37.0	27.0	37.0	14.0	37.0
74-75	31.498321443558538	37.0	27.0	37.0	14.0	37.0
76-77	31.475451112043643	37.0	27.0	37.0	14.0	37.0
78-79	31.53378094838439	37.0	27.0	37.0	14.0	37.0
80-81	31.435585396558956	37.0	27.0	37.0	14.0	37.0
82-83	31.381871590432226	37.0	27.0	37.0	14.0	37.0
84-85	31.506924045321025	37.0	27.0	37.0	14.0	37.0
86-87	31.23268988669744	37.0	27.0	37.0	14.0	37.0
88-89	31.077213596307175	37.0	27.0	37.0	14.0	37.0
90-91	31.123373898447333	37.0	27.0	37.0	14.0	37.0
92-93	30.89613932018464	37.0	27.0	37.0	14.0	37.0
94-95	31.007763323541752	37.0	27.0	37.0	14.0	37.0
96-97	30.89949643306756	37.0	27.0	37.0	14.0	37.0
98-99	30.604490138480905	37.0	27.0	37.0	14.0	37.0
100-101	30.744859420898027	37.0	27.0	37.0	14.0	37.0
102-103	30.722198908938314	37.0	27.0	37.0	14.0	37.0
104-105	30.587075115400754	37.0	27.0	37.0	14.0	37.0
106-107	30.22303818715904	37.0	22.0	37.0	14.0	37.0
108-109	30.10847671002937	37.0	22.0	37.0	14.0	37.0
110-111	29.971254720939992	37.0	22.0	37.0	14.0	37.0
112-113	29.975660931598824	37.0	22.0	37.0	14.0	37.0
114-115	29.956986991187577	37.0	22.0	37.0	14.0	37.0
116-117	29.903063365505666	37.0	22.0	37.0	14.0	37.0
118-119	29.80109106168695	37.0	22.0	37.0	14.0	37.0
120-121	29.678766261015525	33.0	22.0	37.0	14.0	37.0
122-123	29.46936634494335	33.0	22.0	37.0	14.0	37.0
124-125	28.212127570289553	33.0	18.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	2.0
19	17.0
20	45.0
21	66.0
22	54.0
23	47.0
24	34.0
25	66.0
26	68.0
27	67.0
28	113.0
29	106.0
30	123.0
31	128.0
32	169.0
33	213.0
34	270.0
35	364.0
36	429.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.179680940386234	21.368597816960538	7.8085642317380355	24.6431570109152
2	17.582878724297103	23.583718002517834	31.26311372219891	27.570289550986153
3	18.88375996642887	27.738145195132187	30.2559798573227	23.12211498111624
4	14.813260595887536	16.785564414603442	35.83718002517834	32.563994964330675
5	15.02308015107008	34.24255140579102	29.752412924884595	20.9819555182543
6	27.3185060847671	34.87201007133865	19.597146454049515	18.21233738984473
7	23.877465379773394	34.11665967268149	22.324800671422576	19.681074276122533
8	24.00335711288292	31.68275283256399	23.49979018044482	20.814099874108265
9	24.506924045321025	22.53462022660512	21.065883340327318	31.892572387746537
10-11	25.372507869884576	26.61070304302204	24.197271773347325	23.819517313746065
12-13	25.996642887117076	26.18548048678137	25.32522031053294	22.49265631556861
14-15	24.63281577843055	25.702895509861516	24.48594208980277	25.178346621905163
16-17	23.56273604699958	26.877885018883763	25.19932857742341	24.360050356693243
18-19	23.730591691145612	25.95467897608057	27.528325639949642	22.786403692824173
20-21	23.667645824590853	24.63281577843055	28.367603860679814	23.331934536298782
22-23	24.506924045321025	25.49307595467898	26.77297524129249	23.22702475870751
24-25	22.366764582459083	26.017624842635335	27.69618128409568	23.919429290809905
26-27	23.059169114561477	26.731011330255978	26.62610155266471	23.583718002517834
28-29	22.85414480587618	25.20461699895068	27.07240293809024	24.868835257082896
30-31	22.09399916072178	24.92656315568611	29.10197230381872	23.877465379773394
32-33	22.429710449013847	27.486361728913135	26.458245908518673	23.625681913554345
34-35	24.276122534620225	24.255140579101973	26.437263953000418	25.03147293327738
36-37	23.059169114561477	25.702895509861516	26.751993285774233	24.48594208980277
38-39	25.26227444397818	23.688627780109105	26.33235417540915	24.716743600503566
40-41	23.625681913554345	25.388166177087708	26.0805707091901	24.905581200167855
42-43	23.940411246328157	24.590851867394043	26.961812840956778	24.506924045321025
44-45	23.56273604699958	24.318086445656736	26.33235417540915	25.78682333193454
46-47	23.52077213596307	24.024339068401172	27.045740663029793	25.409148132605957
48-49	24.66414777497901	24.307304785894207	25.86062132661629	25.167926112510496
50-51	24.33906840117499	23.394880402853545	25.052454888795634	27.21359630717583
52-53	24.40201426772975	24.171212757028957	26.37431808644566	25.052454888795634
54-55	23.877465379773394	25.80780528745279	25.744859420898024	24.569869911875784
56-57	23.289970625262274	25.26227444397818	25.80780528745279	25.639949643306757
58-59	23.541754091481327	24.98950902224087	25.702895509861516	25.765841376416283
60-61	24.171212757028957	25.472093999160723	24.821653378094837	25.535039865715486
62-63	22.954259336970207	24.506924045321025	25.639949643306757	26.898866974402015
64-65	24.066302979437683	24.653797733948803	25.052454888795634	26.227444397817877
66-67	24.29710449013848	25.241292488459926	24.590851867394043	25.870751154007554
68-69	23.961393201846413	24.73772555602182	25.6189676877885	25.681913554343268
70-71	22.912295425933696	24.758707511540077	25.639949643306757	26.68904741921947
72-73	24.611833822912295	25.51405791019723	24.905581200167855	24.96852706672262
74-75	23.929471032745592	25.482787573467675	24.97900923593619	25.608732157850543
76-77	24.653797733948803	24.611833822912295	25.283256399496434	25.451112043642464
78-79	23.70960973562736	24.98950902224087	25.765841376416283	25.535039865715486
80-81	24.066302979437683	24.67477968946706	24.905581200167855	26.353336130927403
82-83	23.331934536298782	24.129248845992446	25.95467897608057	26.584137641628196
84-85	23.25776658270361	25.65071368597817	25.440806045340054	25.65071368597817
86-87	23.82451721242653	25.608732157850543	25.461796809403864	25.10495382031906
88-89	24.13937867338371	24.66414777497901	25.12594458438287	26.070528967254408
90-91	25.39349422875131	24.637985309548792	25.3305351521511	24.637985309548792
92-93	23.834523309533807	24.212515749685007	25.493490130197394	26.45947081058379
94-95	23.583718002517834	24.73772555602182	25.535039865715486	26.143516575744858
96-97	23.101133025597985	26.227444397817877	24.96852706672262	25.702895509861516
98-99	24.506924045321025	24.863617289131348	24.590851867394043	26.038606798153584
100-101	23.604699958036086	25.49307595467898	25.136382710868656	25.765841376416283
102-103	22.954259336970207	25.91271506504406	25.346202266051193	25.78682333193454
104-105	23.730591691145612	25.849769198489298	25.535039865715486	24.884599244649603
106-107	23.20604280318926	25.639949643306757	26.18548048678137	24.96852706672262
108-109	22.89131347041544	25.556021821233738	25.26227444397818	26.290390264372636
110-111	22.870331514897188	27.297524129248846	24.464960134284517	25.36718422156945
112-113	23.583718002517834	26.68904741921947	24.318086445656736	25.409148132605957
114-115	23.248006714225767	28.010910616869495	23.185060847671004	25.556021821233738
116-117	23.982375157364665	27.423415862358368	23.79353755770038	24.800671422576585
118-119	24.412258606213268	27.204030226700255	22.921914357682617	25.461796809403864
120-121	22.807385648342425	28.367603860679814	23.457826269408308	25.36718422156945
122-123	23.667645824590853	27.69618128409568	24.360050356693243	24.276122534620225
124-125	23.12211498111624	27.65421737305917	23.982375157364665	25.241292488459926
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	2.0
4	3.0
5	3.0
6	1.5
7	1.5
8	2.5
9	4.0
10	6.0
11	5.5
12	4.5
13	5.0
14	4.5
15	3.5
16	3.5
17	5.5
18	6.0
19	6.0
20	7.5
21	6.0
22	4.0
23	3.5
24	5.0
25	6.0
26	5.0
27	5.5
28	8.5
29	12.0
30	13.5
31	18.5
32	21.0
33	22.5
34	25.0
35	27.5
36	28.0
37	27.0
38	29.0
39	35.5
40	41.0
41	38.0
42	40.5
43	53.0
44	64.0
45	64.0
46	71.5
47	83.5
48	98.5
49	101.5
50	94.0
51	107.0
52	121.5
53	115.0
54	105.0
55	101.5
56	87.0
57	78.5
58	83.0
59	82.5
60	67.0
61	51.0
62	45.0
63	38.0
64	31.0
65	24.5
66	20.5
67	16.0
68	10.0
69	8.5
70	8.0
71	8.0
72	8.5
73	10.5
74	8.0
75	3.5
76	2.5
77	2.5
78	2.5
79	2.0
80	1.5
81	0.5
82	0.5
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0419639110365086
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0209819555182543
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0209819555182543
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0419639110365086
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0419639110365086
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0419639110365086
86-87	0.0419639110365086
88-89	0.0419639110365086
90-91	0.0209819555182543
92-93	0.0839278220730172
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0
106-107	0.0
108-109	0.0
110-111	0.0
112-113	0.0
114-115	0.0
116-117	0.0
118-119	0.0419639110365086
120-121	0.0
122-123	0.0
124-125	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2383.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.46076374318086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.97912039093735	91.60721779269828
2	1.9991115059973346	3.776751993285774
3	0.48867170146601513	1.384809064204784
4	0.13327410039982232	0.5035669324381032
5	0.13327410039982232	0.629458665547629
6	0.0888494002665482	0.5035669324381032
7	0.0444247001332741	0.2937473772555602
8	0.0888494002665482	0.6714225765841376
9	0.0	0.0
>10	0.0444247001332741	0.629458665547629
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTTAATGGGCAGGGAGAACAGGTAGATCTCCTCCAAGGACTTGATTTTC	15	0.629458665547629	No Hit
CCAGCCCGAGTCTGCTTCTGCACTGGCATGATTTTCAGAACCTCATCCTT	8	0.3357112882920688	No Hit
ATTTCTTAAAGAATTGAGTACAAAAAACCTTACATAAATTAAAGAATGAA	8	0.3357112882920688	No Hit
GGGTTTATTGTACACGAGCTACACTTGGCCACAAGAGAACAGAGCTAGTC	7	0.2937473772555602	No Hit
CATTTGTGCGATGCCTCGGTTGGTGAGGATGTAGCGGGCGTGGATCAACC	6	0.2517834662190516	No Hit
GTTTTGGTTGTAAGAAAGGCGGTCATTTCTCCAGGCGATCGCGGCGAGGT	6	0.2517834662190516	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.20981955518254303	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.20981955518254303	No Hit
CCACTGTACCGTATGCGAATCTTTGCACCCAACCATGTGGTGGCCAAGTC	5	0.20981955518254303	No Hit
CTTCTTGGCCGCCTGGCGGCCATTGTGGCCAAGCAGGTACTTCTGGGCCG	4	0.1678556441460344	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4	0.1678556441460344	No Hit
GTAAAGTAACAATTCTTGATATCACGTGCCTCTTATGATCCATCTGAAAA	4	0.1678556441460344	No Hit
GAAGGAGTGGTAACTGGTTCAGGAACCCTTGCCAGCCTCAGGAAATCCTG	3	0.1258917331095258	No Hit
GCAGCACACAGTCCGATAGCTTGTCCCGGCCCTCAGCATCCCTCACTTCT	3	0.1258917331095258	No Hit
CCTTTATAAGTGATGGCTGCCATCAGGAAGAAACTGGTGATTGTTGGTGA	3	0.1258917331095258	No Hit
CTCATAGTCTTCAGCAAGGACCAGTTCCCAGAGGTCTATGTGCCCACGGT	3	0.1258917331095258	No Hit
GGGTGTTCTTCTCCTGCGTCTCGGTTTTCTTCAGCTTGGCCTTATCGAAG	3	0.1258917331095258	No Hit
GGATCACTTGGCCCTTCCTCTTCTTGTCGCCTCCCAGTTCAAAATGCTTG	3	0.1258917331095258	No Hit
GTATTTGACATCATATTCTTCAATCCCACAGTACCATTCCAGATAGACTA	3	0.1258917331095258	No Hit
GCACACAGTCCGATAGCTTGTCCCGGCCCTCAGCATCCCTCACTTCTGCT	3	0.1258917331095258	No Hit
GCTGCCGAAGATGGCGGAGGGGCAGGTTCTGGTATTGGATGGCCGAGGCC	3	0.1258917331095258	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.1258917331095258	No Hit
GAATTGAGTACAAAAAACCTTACATAAATTAAAGAATGAATACATTTACA	3	0.1258917331095258	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0629458665547629	0.0
34-35	0.0	0.0	0.0	0.0839278220730172	0.0
36-37	0.0	0.0	0.0	0.1468736886277801	0.0
38-39	0.0	0.0	0.0	0.2517834662190516	0.0
40-41	0.0	0.0	0.0	0.2937473772555602	0.0
42-43	0.0	0.0	0.0	0.31472933277381454	0.0
44-45	0.0	0.0	0.0	0.3566932438103231	0.0
46-47	0.0	0.0	0.0	0.46160302140159465	0.0
48-49	0.0	0.0	0.0	0.46160302140159465	0.0
50-51	0.0	0.0	0.0	0.5245488879563576	0.0
52-53	0.0	0.0	0.0	0.5455308434746118	0.0
54-55	0.0	0.0	0.0	0.6084767100293748	0.0
56-57	0.0	0.0	0.0	0.6294586655476291	0.0
58-59	0.0	0.0	0.0	0.6714225765841376	0.0
60-61	0.0	0.0	0.0	0.7973143096936635	0.0
62-63	0.0	0.0	0.0	0.8812421317666806	0.0
64-65	0.0	0.0	0.0	0.9441879983214436	0.0
66-67	0.0	0.0	0.0	0.9651699538396978	0.0
68-69	0.0	0.0	0.0	1.0071338648762065	0.0
70-71	0.0	0.0	0.0	1.028115820394461	0.0
72-73	0.0	0.0	0.0	1.0700797314309693	0.0
74-75	0.0	0.0	0.0	1.1540075535039866	0.0
76-77	0.0	0.0	0.0	1.2169534200587495	0.0
78-79	0.0	0.0	0.0	1.3428451531682752	0.0
80-81	0.0	0.0	0.0	1.384809064204784	0.0
82-83	0.0	0.0	0.0	1.447754930759547	0.0
84-85	0.0	0.0	0.0	1.5526647083508183	0.0
86-87	0.0	0.0	0.0	1.6156105749055811	0.0
88-89	0.0	0.0	0.0	1.6785564414603442	0.0
90-91	0.0	0.0	0.0	1.80444817456987	0.0
92-93	0.0	0.0	0.0	1.8883759966428872	0.0
94-95	0.0	0.0	0.0	1.9932857742341588	0.0
96-97	0.0	0.0	0.0	2.0562316407889214	0.0
98-99	0.0	0.0	0.0	2.1401594628619387	0.0
100-101	0.0	0.0	0.0	2.2870331514897186	0.0
102-103	0.0	0.0	0.0	2.370960973562736	0.0
104-105	0.0	0.0	0.0	2.412924884599245	0.0
106-107	0.0	0.0	0.0	2.580780528745279	0.0
108-109	0.0	0.0	0.0	2.7066722618548047	0.0
110-111	0.0	0.0	0.0	2.8745279060008393	0.0
112-113	0.0	0.0	0.0	3.000419639110365	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE