FastQCFastQC Report
Sun 1 Jan 2017
ERR1142396_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142396_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277419
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41621.5002577328877977No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA26310.9483849339807295No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21810.7861754241778681No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG12470.4495005749425959No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11570.4170586729820236No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT9730.35073300675152025No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC9070.3269422786471006No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT8450.3045934128520397No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC8140.2934189799545092No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG7960.2869305995623948No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC7400.26674452723137204No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5670.20438398235160535No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC5380.19393048060875426No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG4970.1791513919378269No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC4720.1701397525043346No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT4440.16004671633882323No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA4200.15139554248267062No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC4110.14815135228661339No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA3650.13156993572898756No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC3490.1258024864915525No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3470.1250815553368731No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC3250.11715131263539988No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT3030.10922106993392665No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG2780.10020943050043436No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTCC150.004235899559.49818880-81
TAGACGA300.00423973459.476664
GTACAAG3150.052.9639741
GGGCGTA350.00785808250.8879287
GTACAAA2900.045.202011
GGATTGG853.5198173E-641.915278
GAACAAA5550.039.7229771
TACAAGG2600.038.8885842
GGGATTG1102.0650506E-532.3832247
TAGGCTG1004.4449107E-429.732955
TATCCTC800.005370273329.7329485
CGGGAGT2006.103801E-826.7644964
TGGGAGA3200.025.9693556
ATGGGAC5050.025.9059375
TACAAGA2558.785719E-1025.6565992
CGCAGAA1401.0558915E-425.4899982
AGTACAA2701.7043931E-924.231232
GAGTACT2750.023.83810812-13
ATTAAGG1300.0020529222.8756393
GGGAGAT2102.8310096E-622.6168567
Produced by FastQC (version 0.11.5)