FastQCFastQC Report
Sun 1 Jan 2017
ERR1142363_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142363_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69675
Sequences flagged as poor quality0
Sequence length125
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3740.536777897380696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3250.46645138141370646No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2640.3789020452099031No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2370.3401506996770721No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1710.24542518837459634No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.18083961248654468No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1250.1794043774668102No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACAC1220.17509867240760674No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1130.16218155722999641No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATAC1040.14926444205238606No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1040.14926444205238606No Hit
GCTGCTAGCTGGAGCCACCCGCAGTTCGAAAAAGTGAGCAAGGGCGAGGA1030.1478292070326516No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCTCTTATAC980.14065303193397918No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACAC950.13634732687477574No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT910.13060638679583783No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN840.12055974165769644No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC810.116254036598493No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.11051309651955508No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG750.1076426264800861No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC710.10190168640114819No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACA700.1004664513814137No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAAGA750.087.2334062
GTACATA454.1283784E-879.647141
TTGCGTT304.2440406E-579.30315
TACATAG359.1093185E-567.974082
TTTTGCG453.9585266E-666.0859153
GTACAAA753.0559022E-1063.7177121
GTACAAG1600.063.4688151
AGGGAGG150.004217022659.54162618-19
AGAACAG150.004235113559.47732520-21
TTTGCGT401.7636752E-459.477324
ACAAGAA1300.054.9021453
GAACAAA2200.054.304871
ACATAGG453.1561294E-452.8687323
CACAGAG2150.050.0471849
CAAGAAA1350.048.4630054
GAAAAGC802.3026932E-644.5118527
AGTACTC705.4111988E-542.4838035
GAGTACT1900.042.47425512-13
AGTACAA1151.3978934E-841.375532
GTACTTT2100.041.27569214-15
Produced by FastQC (version 0.11.5)