FastQCFastQC Report
Sun 1 Jan 2017
ERR1142363_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142363_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69675
Sequences flagged as poor quality0
Sequence length125
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53427.6670254754216005No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50057.18335127377108No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17372.4930032292787945No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15912.28345891639756No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7541.082167204879799No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5450.7822030857552925No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3380.4851094366702548No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3340.47936849659131686No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2720.390383925367779No Hit
GCGTTGATACCACTGCTTGTATCAACGCAGAGTACTTTTTTTTTTTTTTT2690.3860782203085755No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2670.3832077502691066No Hit
GATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2530.3631144599928238No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.3573735199138859No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2370.3401506996770721No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.33871546465733765No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.33871546465733765No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1960.28130606386795837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1950.2798708288482239No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1940.2784355938284894No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1770.2540365984930032No Hit
GTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1600.22963760315751705No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1590.22820236813778255No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1550.22246142805884464No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1540.22102619303911017No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT1540.22102619303911017No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1460.2095443128812343No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1440.20667384284176535No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT1340.19232149264442053No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1340.19232149264442053No Hit
GCTGCTAGCTGGAGCCACCCGCAGTTCGAAAAAGTGAGCAAGGGCGAGGA1300.1865805525654826No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACAC1260.18083961248654468No Hit
GCTTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.1779691424470757No Hit
GCGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.17509867240760674No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1160.16648726228919986No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCTCTTATAC1100.15787585217079297No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT1090.15644061715105848No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1040.14926444205238606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1010.14495873699318262No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC970.1392177969142447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC930.13347685683530677No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT910.13060638679583783No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATAC900.12917115177610333No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT880.1263006817366344No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT860.12343021169716542No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC860.12343021169716542No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT850.12199497667743094No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC840.12055974165769644No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACAC830.11912450663796198No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.116254036598493No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC790.11338356655902404No Hit
GCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.1090778614998206No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT760.1090778614998206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT740.10620739146035163No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT720.10333692142088267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT720.10333692142088267No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG710.10190168640114819No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACACA700.1004664513814137No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC700.1004664513814137No Hit
CTGCTAGCTGGAGCCACCCGCAGTTCGAAAAAGTGAGCAAGGGCGAGGAG700.1004664513814137No Hit
GCTTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.1004664513814137No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCAGA500.0119.03161
GCATCAA205.6492445E-6119.03161
CATCAAC205.6694807E-6118.946162
CTTGGTA251.1982411E-7118.946162
GATCAAC800.0104.152651
GCTTGGT303.5372068E-799.193011
GCAACGC358.855841E-785.0225751
GTCAACG900.079.354411
AGGTATC304.2456297E-579.297432
GTGTTGA250.002052400371.36776
CGTACTT300.00422745559.4730762
CAGTGCT150.00423633759.47307626-27
GGTATCA22400.053.9361951
GCTTGTA504.4305832E-447.612641
GGAGCCC255.3282554E-447.57846562-63
TTGGTAT653.4953533E-545.7485243
GTGGCCC300.001307465639.64871660-61
CAGTGGC300.001307465639.64871626-27
GTATCAA34300.038.8674661
TTTGATA801.1840869E-437.1973761
Produced by FastQC (version 0.11.5)