FastQCFastQC Report
Fri 29 Apr 2016
ERR1307267.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307267.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4803402
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT191670.39902968770883634No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT183710.3824580994886541No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT123630.25738008186697675No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA76420.1590955743450163No Hit
GAGTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATA63920.13307235163744363No Hit
GGTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACAT58330.12143476644261714No Hit
TATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATTT55480.11550147166529057No Hit
GCTTATAGCAATTTCAAGCGACTTAAATGCATATGGTGTTGTA52390.10906853101197858No Hit
GTGTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCC52300.10888116380848407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55250.032.3122181
CTTATAG14750.021.4474582
GTATCAA84800.020.965212
GCTTATA15250.020.8655741
CGACTTA14600.019.26027319
TTATAGC17050.018.8797663
GCGACTT14950.018.80936418
AAGCGAC15550.018.08360316
CTTATAC27200.017.81985337
CAAGCGA16800.016.73809415
TATAGCA20350.016.5454544
ATAGCAA19050.016.2178485
AGCGACT17250.016.19420217
TCTTATA44800.016.14620637
GTACCGT2100.015.8571426
TCAAGCG18250.015.50958814
TATACCG3200.015.031256
TAGCAAT20400.014.8725496
AGTACCG4150.014.265065
GTATAGG5750.014.1565221
Produced by FastQC (version 0.11.5)