FastQCFastQC Report
Fri 29 Apr 2016
ERR1307266.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307266.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3839213
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT51510.1341681224771848No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT48040.12512981176089996No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA46390.12083205594479911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37900.027.8720321
GCTTATA9400.019.2872331
CTTATAC22000.019.08863637
CTTATAG9400.018.8936162
GTATCAA56100.018.6319082
TTATAGC11000.017.3227273
GCGACTT8950.017.15642518
AAGCGAC8850.017.14124516
CGACTTA8650.017.10982719
TATACTG4050.015.9876555
TCTTATA36700.015.67711137
GAGCGGT7650.015.2352946
AGCGACT10550.014.72985717
GTCCTAA6350.014.5669291
ATCGATA5000.014.43000123
TATAGCA13750.013.9927274
CGTATAG800.006302163513.8753
TAATCGA5200.013.87521
AGCGGTC7750.013.8451627
CAAGCGA11150.013.77130115
Produced by FastQC (version 0.11.5)