FastQCFastQC Report
Fri 29 Apr 2016
ERR1307265.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307265.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7351954
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT370890.504478129215716No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT341150.46402629831470654No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT233100.3170585670149732No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA128620.17494668764249613No Hit
GAGTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATA99130.1348349023946559No Hit
GGTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACAT98750.1343180330018387No Hit
TATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATTT94210.1281428039402858No Hit
GTGTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCC89380.12157312192105664No Hit
GCTTATAGCAATTTCAAGCGACTTAAATGCATATGGTGTTGTA88120.1198592918290838No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79080.10756324101048512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97650.031.6763951
CTTATAG23250.022.120432
GCTTATA24450.021.5644171
GTATCAA153200.020.1664492
TTATAGC26450.020.0037823
GCGACTT24050.019.2307718
CGACTTA24400.018.87909719
AAGCGAC24800.018.87298416
CTTATAC49100.018.6883937
TATAGCA31750.017.1307094
AGCGACT27850.016.9389617
CAAGCGA28050.016.68627515
ATAGCAA30750.016.3642275
TAGCAAT32750.015.4778626
TCTTATA76700.015.09908737
TCAAGCG32300.014.37616214
GACTTAA33100.014.30815720
CTACACT12100.014.0661154
GCAATTT32950.013.9241278
AGCAATT35500.013.7577467
Produced by FastQC (version 0.11.5)