FastQCFastQC Report
Fri 29 Apr 2016
ERR1307264.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307264.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176920
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATTT3080.1740899841736378No Hit
GGTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACAT2750.15543748586931946No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA2330.1316979425729143No Hit
GTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATT2210.12491521591679855No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGT2150.12152385258874067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGC355.595466E-737.03
CTTATAG401.5899568E-632.3752
GTTACTC250.005483936529.59999818
CGTTACT250.005483936529.59999817
GGTATCA2250.028.7777791
ATAGCAA405.912191E-527.7500025
TAGCAAT405.912191E-527.7500026
GCTTATA451.3170391E-424.6666681
GCAATTT451.3170391E-424.6666688
ATGCTTA400.001924769123.1250026
GTCTAGG400.001924769123.1250021
GTATCAA2800.022.4642852
TATAGCA502.6895994E-422.1999994
AATTTCA450.003813215820.55555710
TCTTAGA450.003813215820.5555572
CCTACGA450.003813215820.5555572
TTGGACA555.119491E-420.1818184
ATCCGGA555.119491E-420.1818181
ACGAGTA555.119491E-420.1818185
CTTAGAG555.119491E-420.1818183
Produced by FastQC (version 0.11.5)