FastQCFastQC Report
Fri 29 Apr 2016
ERR1307263.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307263.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7607717
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT167940.22074953629321387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT164980.21685875013489594No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA130360.17135232553997473No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT116020.15250304394866424No Hit
GCTTATAGCAATTTCAAGCGACTTAAATGCATATGGTGTTGTA103780.13641411740210632No Hit
GAGTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATA99870.13127459919973364No Hit
TATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATTT96320.12660828471931856No Hit
GGTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACAT93620.12305925680463667No Hit
GTGTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCC88530.11636868195806968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72100.031.5603331
GCTTATA28450.022.0439361
CGACTTA25500.021.32941219
CTTATAG29100.021.297252
GCGACTT25850.021.04061918
AAGCGAC28550.020.08756616
TTATAGC30850.020.0291733
GTATCAA116100.019.551682
TATAGCA32950.019.089534
ATAGCAA32250.018.9302335
AGCGACT30400.018.62171217
CTTATAC47600.018.38340437
TAGCAAT34950.017.7324756
CAAGCGA32600.017.64877315
TCAAGCG34950.016.40915714
GCAATTT36600.015.9221318
AGCAATT38900.015.3136237
TCTTATA79550.015.2790737
CTATACA7200.014.9027794
TATACCG6150.014.7398396
Produced by FastQC (version 0.11.5)