FastQCFastQC Report
Fri 29 Apr 2016
ERR1307262.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307262.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1765214
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA50480.28597099275215354No Hit
TATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATTT47180.26727637555559836No Hit
GGTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACAT42540.24099061077013895No Hit
GAGTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATA41460.2348723724149027No Hit
GCTTATAGCAATTTCAAGCGACTTAAATGCATATGGTGTTGTA40880.23158665181672025No Hit
GTGTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCC35760.20258166998448915No Hit
GTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATT28650.16230326747918383No Hit
GTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATACT21020.11907904650654255No Hit
CTCTACAACACCATATGCATTTAAGTCGCTTGAAATTGCTATA19230.10893863293628989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19550.030.9437331
GCTTATA10400.024.9038471
CTTATAG10450.024.4306222
GCGACTT10500.023.60952618
TTATAGC11150.023.2286993
ATAGCAA11350.022.8193845
CGACTTA10800.022.61111319
AAGCGAC11150.022.56502216
TATAGCA12150.021.9259264
AGCGACT11600.021.3706917
CAAGCGA12300.020.75609815
GCAATTT12200.020.6229528
TAGCAAT12600.020.1150786
GTATCAA30200.020.0927162
AGCAATT13000.019.780777
GACTTAA12450.019.76305220
TCAAGCG13600.018.90808714
AATTTCA13550.018.56826610
TCGCTAG701.2196667E-418.528
TTTCAAG14400.018.37152712
Produced by FastQC (version 0.11.5)