FastQCFastQC Report
Fri 29 Apr 2016
ERR1307261.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307261.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8380317
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT426210.5085845797957285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT410050.48930129970023806No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT291620.34798206320834885No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA195770.2336069148696881No Hit
GAGTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATA163620.1952432109668405No Hit
GGTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACAT142620.17018449302096805No Hit
GCTTATAGCAATTTCAAGCGACTTAAATGCATATGGTGTTGTA138000.16467157507287614No Hit
TATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATTT137750.16437325700209193No Hit
GTGTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCC137490.16406300620847636No Hit
GTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATT98040.11698841463873026No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89490.10678593661791076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126450.030.2846981
GCTTATA37650.022.4063761
CTTATAG38050.021.8304882
GCGACTT37800.020.1150818
CGACTTA37350.019.91164619
AAGCGAC39300.019.67684416
TTATAGC42450.019.4369853
GTATCAA198700.019.20762
CAAGCGA40400.019.1410915
AGCGACT41400.018.63405817
TATAGCA46400.018.1810364
ATAGCAA45400.018.0925125
TCAAGCG43950.017.51080914
CTTATAC51600.016.92248237
TAGCAAT49100.016.7668026
GCAATTT47350.016.2534318
AGCAATT50850.015.6440527
GACTTAA49000.015.32857120
TCTTATA81100.014.576447537
GTACTAG6350.014.2755911
Produced by FastQC (version 0.11.5)