FastQCFastQC Report
Fri 29 Apr 2016
ERR1307260.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307260.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6759646
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT168180.2488000111248429No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT166390.24615194345976107No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT117260.17347062257402238No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA69910.10342257567925894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57750.030.6891781
GCTTATA18900.021.2407421
CTTATAG18700.021.1711222
GCGACTT16500.020.74242418
CGACTTA16700.020.050919
GTATCAA92550.019.109672
AAGCGAC18300.018.70218516
AGCGACT18800.018.59840417
CTTATAC42550.018.43478237
TTATAGC21500.018.3279083
TATAGCA23850.017.53044
CAAGCGA20900.016.37559715
TCAAGCG22200.015.66666714
TAGCAAT24750.015.6222236
ATAGCAA24450.015.2842545
TCTTATA68300.014.62664737
ATCGATA7900.014.51898723
TATACCG4750.014.0210526
GCAATTT25550.013.974568
TCTATAC7900.013.8164563
Produced by FastQC (version 0.11.5)