FastQCFastQC Report
Fri 29 Apr 2016
ERR1307259.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307259.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4836384
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT84870.17548234383374026No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT82360.17029251606158652No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA58280.1205032520163825No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT58160.12025513276034328No Hit
GCTTATAGCAATTTCAAGCGACTTAAATGCATATGGTGTTGTA51690.10687736953889518No Hit
GAGTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATA51070.1055954200493592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41450.032.2691
GCTTATA15100.021.44041
CTTATAG15300.020.7973862
GTATCAA64400.020.7406852
GCGACTT14350.020.62717818
CGACTTA14250.020.38245619
AAGCGAC14900.019.86577216
TTATAGC16850.019.5430283
AGCGACT17000.017.52058817
TATAGCA19550.017.0332474
CAAGCGA18150.016.81818415
ATAGCAA18450.016.6449875
CTTATAC27150.016.14917237
TAGCAAT19700.015.8705596
TCAAGCG19950.015.48621614
TCTTATA44900.015.28619237
TATACCG3000.014.86
GACTTAA20500.014.70975620
GCAATTT19900.014.6884428
GTATTAG7650.014.0261441
Produced by FastQC (version 0.11.5)