FastQCFastQC Report
Fri 29 Apr 2016
ERR1307257.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307257.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5887267
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT187420.3183480552181513No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT181380.3080886258428571No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA169630.2881302988296607No Hit
TATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATTT131850.22395790780340014No Hit
GAGTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATA127460.21650113711506547No Hit
GGTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACAT126480.21483652771311373No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT119030.202182099096236No Hit
GTGTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCC108110.1836335943316313No Hit
GCTTATAGCAATTTCAAGCGACTTAAATGCATATGGTGTTGTA107520.1826314315284155No Hit
GTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATT83770.14229013224642267No Hit
GTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATACT60130.10213567687689382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73250.032.35291
GCTTATA27300.022.2271061
TTATAGC28400.021.4964793
CTTATAG28000.021.0767862
CTTATAC35900.020.45821637
GTATCAA116100.020.4280822
GCGACTT26650.020.06191418
TATAGCA31050.019.7814
CGACTTA27300.019.2454219
AAGCGAC28100.019.15836116
ATAGCAA30800.019.100655
TAGCAAT31350.018.5885166
AGCGACT29750.018.03361317
CAAGCGA31450.017.11764715
TATATCG1901.8189894E-1216.5526315
TCAAGCG33000.016.42575614
GCAATTT34850.016.0315658
AGCAATT36800.015.7350537
TCTTATA63050.015.60983437
TAGATCG1902.7284841E-1115.5789485
Produced by FastQC (version 0.11.5)