FastQCFastQC Report
Fri 29 Apr 2016
ERR1307256.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307256.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14002482
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT822120.5871244826452909No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT807280.5765263615407612No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT581400.4152121031114341No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220600.15754349835979078No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA146420.10456717601922288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA167850.032.59131
GTATCAA277300.019.6141362
GCTTATA35600.019.4353921
CTTATAG35750.018.8363632
CTTATAC121600.018.71299437
CGACTTA31850.018.18053219
GCGACTT33000.017.88333318
AAGCGAC34000.017.73823516
TTATAGC40100.017.208233
AGCGACT39200.015.43239817
TATAGCA46700.015.291224
CAAGCGA42450.014.73027115
TCTTATA196250.014.3380937
ATAGCAA48800.014.026645
GTACTAG9950.013.9447231
TAGCAAT50450.013.6412296
GTATTAG26450.013.6389421
TCAAGCG46950.013.20021314
ATCAACG423450.012.8838124
TATCAAC430300.012.7432033
Produced by FastQC (version 0.11.5)