FastQCFastQC Report
Fri 29 Apr 2016
ERR1307255.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307255.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251354
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATTT6720.26735202145181697No Hit
GGTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACAT6010.23910500728056844No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA4640.18460020528815932No Hit
GTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATT4110.16351440597722733No Hit
GAGTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATA3940.1567510363869284No Hit
GTGTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCC3640.14481567828640085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACAC308.286152E-637.03
CCTATAC250.005488278329.5999983
GGTATCA2700.027.4074061
ATCCTAT358.8500476E-426.4285721
TAGAGCT358.8500476E-426.4285724
GGTATGG400.001927014423.1250021
CGGGAAC502.693972E-422.19999917
ACTGTGT603.711397E-521.5833328
GTTCTAG450.003817620520.5555571
TTAAGGT450.003817620520.5555574
TAAGGTG450.003817620520.5555575
GCTTATA555.1277445E-420.1818181
TAGAACT656.874747E-519.9230774
CTACACA609.2094735E-418.54
TAGAAGT500.00701997418.4999984
CTTTGTA500.00701997418.4999982
GTATAAG500.00701997418.4999981
AGTAGGA500.00701997418.4999982
GTTATGG500.00701997418.4999981
GTATCAA4050.018.2716052
Produced by FastQC (version 0.11.5)