FastQCFastQC Report
Fri 29 Apr 2016
ERR1307254.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307254.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6946551
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT648030.9328802163836414No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT614160.8841222068332902No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT437040.6291467521076286No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA241890.34821597077456135No Hit
GAGTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATA214500.30878633151905166No Hit
GGTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACAT184390.265441080041016No Hit
TATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATTT179450.2583296372545167No Hit
GTGTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCC161580.2326046407778479No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150330.2164095534604151No Hit
GCTTATAGCAATTTCAAGCGACTTAAATGCATATGGTGTTGTA139730.20115018229910067No Hit
GTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATT129290.18612114126852303No Hit
CTCTACAACACCATATGCATTTAAGTCGCTTGAAATTGCTATA90670.13052520596192269No Hit
GTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATACT89560.128927290679936No Hit
CTACAACACCATATGCATTTAAGTCGCTTGAAAAAAAAAAAAA86930.1251412391559495No Hit
CTCTACAACACCATATGCATTTAAGTCGCTTGAAAAAAAAAAA80740.11623034222306868No Hit
CTACAACACCATATGCATTTAAGTCGCTTGAAATTGCTATAAG79470.11440209681034517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145100.032.2315641
GCTTATA36400.022.159341
CTTATAG36350.021.782672
TTATAGC38050.021.0039423
GTATCAA230600.020.2810082
GCGACTT36750.019.93469418
TATAGCA41250.019.3745464
AAGCGAC38200.019.37172916
CGACTTA37550.019.21438219
ATAGCAA42050.018.5225
CAAGCGA40550.018.38594215
CTTATAC50750.017.97142837
TAGCAAT43700.017.780326
AGCGACT41250.017.7617
GCAATTT44050.016.6730988
TCAAGCG45150.016.5537114
AGCAATT46950.016.194897
TAGTACC8800.015.9772734
CTAGTAC8000.015.7249993
GACTTAA47050.015.5313520
Produced by FastQC (version 0.11.5)