FastQCFastQC Report
Fri 29 Apr 2016
ERR1307253.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307253.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13830287
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT808370.5844925705446316No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT775760.5609138841442697No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT567800.4105482409728735No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA215330.1556945275249892No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208940.1510742329497573No Hit
GAGTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATA181690.13137109880655404No Hit
GGTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACAT161390.11669316768336044No Hit
TATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATTT155690.11257177815615829No Hit
GTGTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCC149010.10774179885059507No Hit
GCTTATAGCAATTTCAAGCGACTTAAATGCATATGGTGTTGTA147030.10631015827798802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA177900.031.4676781
GCTTATA40750.020.3840481
GTATCAA285350.019.5794642
CTTATAG41200.019.3980582
CGACTTA38100.018.59711319
CTTATAC108550.018.40626337
GCGACTT38950.018.33376118
AAGCGAC40550.017.9753416
TTATAGC45950.017.6746463
TATAGCA54650.016.1811524
AGCGACT45800.015.83406217
ATAGCAA53450.015.5060815
CAAGCGA47800.015.40376515
TCTTATA171200.014.4801437
TAGCAAT55200.014.4447466
TCAAGCG51500.014.15339814
GTATTAG28100.013.2330961
GCAATTT56450.013.2072638
CTAGTAC12450.013.0763053
AGCAATT60100.013.02087
Produced by FastQC (version 0.11.5)