FastQCFastQC Report
Fri 29 Apr 2016
ERR1307251.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307251.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270256
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATTT6320.23385234740394292No Hit
GGTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACAT5700.2109111361079865No Hit
GTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATT4060.15022793203481144No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA3840.1420875022201172No Hit
GAGTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATA2790.10323545083180392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3350.032.029851
CTTAATC250.005488998729.63
ACGCATC250.005488998729.629
CTTATAG604.2971806E-827.7500022
TTATAGC556.232003E-726.909093
CGCTCGG358.851777E-426.42857210
GCTTATA659.336327E-825.6153851
CGACTTA601.331091E-624.66666819
TTAGACT451.319557E-424.6666664
ACCGTCT400.001927387923.1258
ATAGCAA652.6702583E-622.769235
GTATCAA5000.021.462
TAGCAAT705.0781327E-621.1428596
TATAGCA806.919672E-720.81254
TACTACA450.00381835420.5555552
AAGCGAC759.222842E-619.73333216
AGCAATT902.1398482E-618.57
CTCGTAT609.211916E-418.534
TTGGAGT500.007021305618.54
TAAAAGT500.007021305618.54
Produced by FastQC (version 0.11.5)