FastQCFastQC Report
Fri 29 Apr 2016
ERR1307250.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307250.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4034309
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41570.10304118995347157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42850.018.0035021
CTTATAC24850.017.9416537
GTACTAA2350.016.5319161
GCGACTT4850.015.63917518
CGACTTA4900.015.1020419
GCTTATA6100.014.8606561
TAGGACG4050.014.1604944
TATACTG6100.013.950825
CTTATAG6900.013.9420292
GCTATAC3850.013.9350643
TCTTATA39650.013.90416137
GTTATAC2304.0017767E-1113.6739133
TATAGCA9050.013.4917134
AAGCGAC5700.013.30701816
TTATAGC7450.013.1610743
CTAATCG1551.8906685E-613.12903226
GTATCAA58650.013.027282
CTAATAC8850.012.7514123
GTGCCGT2204.094545E-912.61363611
GTATATA6600.012.3333331
Produced by FastQC (version 0.11.5)