FastQCFastQC Report
Fri 29 Apr 2016
ERR1307249.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307249.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2522112
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14500.027.303451
GTATCAA21750.017.8620682
CTTATAC15350.016.87296337
GCTTATA4400.015.5568181
GTATTAG5350.015.2149521
TCTTATA23550.015.16135837
TATACTG4300.015.0581415
GTATAGG3200.015.0312511
ATATAAC3350.014.9104483
GCGACTT3250.014.818
AAGCGAC3350.014.35820916
CTTATAG4650.014.322582
TATTAGA3300.014.015152
TATAACT3450.013.9420284
ACCGATA950.001245795713.631577526
AGTACCG1501.3071531E-613.5666685
TAATACT5250.013.3904754
GGTCTAG3350.013.2537321
AGCGACT3500.013.21428617
CGACTTA3500.013.21428619
Produced by FastQC (version 0.11.5)