FastQCFastQC Report
Fri 29 Apr 2016
ERR1307248.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307248.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3530986
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20950.029.4940361
AAGCGAC3900.018.97435816
GTATCAA33400.018.2784422
GCTTATA5500.017.1545451
GCGACTT4350.017.01149618
TCGCTAA1851.8189894E-1217.014
CTTATAC18450.016.94579937
CGACTTA4600.015.68478319
CTTATAG5650.015.389382
GTATAGG5050.015.3861381
TCTTATA30850.015.29173437
TAATACG2550.015.2352944
GTACTAG2800.015.1964281
TATACTG3750.014.8000015
AGCGACT5300.014.660377517
TTATAGC6950.014.1079143
TTTAGCG1854.9094524E-914.026
TGGCGTA2750.013.45454524
GTATAGA6300.013.2142851
TACGAGT3950.013.1139244
Produced by FastQC (version 0.11.5)