FastQCFastQC Report
Fri 29 Apr 2016
ERR1307247.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307247.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3349758
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27550.027.1960071
GCTTATA5400.018.8425921
CTTATAC21450.018.80186537
GTATCAA40100.018.6845382
CTTATAG5850.016.7606832
TCTTATA37800.016.59126937
CGACTTA5400.015.75925919
AAGCGAC5600.015.52678716
GCGACTT5500.015.47272718
GTATACG1101.45344E-515.1363641
GTATATA4450.014.5505611
TATACTG3350.013.805975
ATCAACG54350.013.7856484
TTATAGC8100.013.7037043
TCGCTAA1907.1395334E-913.63157914
TGTACTA2457.2759576E-1213.5918385
CAACGCA55150.013.5521316
TCAACGC55200.013.5398545
CTAGACT4900.013.2142874
TATCAAC58350.013.1893743
Produced by FastQC (version 0.11.5)