FastQCFastQC Report
Fri 29 Apr 2016
ERR1307246.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307246.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433519
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10350.027.7053151
CGTGATA603.7271147E-521.58333224
ATATCGC555.1439466E-420.1818186
GTATCAA14900.019.4932882
TCGCTAA951.6768354E-719.47368414
CTTATAC12000.019.42537
ATACTTA609.2383364E-418.535
TCTATTC902.1529722E-618.53
GTATTAG2750.018.1636371
CTTATAG1750.017.971432
GCTTATA1750.017.971431
GTTAGAC852.7238751E-517.4117643
ATCGATA650.001580191417.07692323
CGACTTA1655.4569682E-1116.81818219
GTATAAA1352.2204404E-816.4444431
TCTAGAC1708.54925E-1116.323533
GTATAGA1708.54925E-1116.323531
CTATGTT2050.016.2439024
CTAGACT2003.6379788E-1215.7254
GCGACTT1659.749783E-1015.6969718
Produced by FastQC (version 0.11.5)