FastQCFastQC Report
Fri 29 Apr 2016
ERR1307245.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307245.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3544567
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20800.026.237981
CTTATAC27600.020.91304237
CGACTTA4100.018.95121819
GCTTATA5000.018.51
CTTATAG4900.017.74492
GCGACTT4800.017.72916818
GTATCAA31150.017.4012832
GTACTAG2450.017.3673481
TCGCTAA1757.2759576E-1216.91428614
TTATAGC5800.015.94827653
GTATTAG5550.015.6666671
GTATTAA2600.015.6538461
TCTTATA47200.015.56038137
GCGTAGA1557.223207E-915.51612951
CGTCTAA1904.5656634E-1014.6052641
TATACAC7700.014.41558437
CCTATAC3600.014.3888883
AAGCGAC5700.014.28070316
TTGCGTA1203.304174E-513.87500122
CTAATAC5600.013.8750013
Produced by FastQC (version 0.11.5)