FastQCFastQC Report
Fri 29 Apr 2016
ERR1307244.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307244.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7320610
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT80360.10977227307560436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT74660.10198603668273545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50950.030.2463191
GTATCAA78850.019.4502222
CTTATAC53650.019.24137937
GCTTATA18400.018.198371
CTTATAG18100.017.988952
GCGACTT15900.017.91823818
CGACTTA16250.017.4184619
TTATAGC20550.016.2043783
TCTTATA89850.015.66889237
CTAGTAC7350.015.6054433
AAGCGAC18650.015.27613916
TATACCG3400.014.6911766
TATAGCA23000.014.3173914
AGCGACT20650.013.88619817
TATACTG9350.013.8502665
TCGCTAA4000.013.412514
ATACCGC3900.013.2820527
CAAGCGA22750.013.17362615
GTATTAG11400.012.9824571
ATAGCAA23550.012.9617835
Produced by FastQC (version 0.11.5)