FastQCFastQC Report
Fri 29 Apr 2016
ERR1307243.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307243.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7790536
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT421660.5412464559563038No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT395160.5072308246826662No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT287910.36956378867898176No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA105700.1356774424763585No Hit
GCTTATAGCAATTTCAAGCGACTTAAATGCATATGGTGTTGTA99680.12795011793796987No Hit
GAGTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATA91130.11697526331949432No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90890.11666719722494062No Hit
TATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATTT90340.11596121242492172No Hit
GGTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACAT88070.11304742061393465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94200.032.1295131
GCTTATA27350.022.118831
CTTATAG26400.022.0738662
GCGACTT25250.020.44158618
CGACTTA25500.020.24117719
TTATAGC29700.019.745793
CTTATAC69400.019.64625537
GTATCAA154350.019.5487542
AAGCGAC27800.019.29856116
TATAGCA35150.017.8421064
AGCGACT29350.017.8381617
ATAGCAA33150.017.3001525
TAGCAAT33900.016.9174046
CAAGCGA31750.016.83936915
TCAAGCG32300.016.72445914
GCAATTT35350.015.3861388
TCTTATA113100.015.37577337
AGCAATT37450.014.819767
CCTATAC7150.014.7482523
TATAAGC21650.014.6120093
Produced by FastQC (version 0.11.5)