FastQCFastQC Report
Fri 29 Apr 2016
ERR1307242.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307242.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8736203
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT392510.44929129966416764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT377910.4325792337929876No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT270220.30931057806234585No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA94220.10785005797140931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98950.030.92371
CGACTTA21100.019.9905219
CTTATAC67550.019.2257637
GCTTATA24350.019.145791
CTTATAG24600.019.1016272
GCGACTT22600.018.9911518
GTATCAA160800.018.983212
AAGCGAC22950.018.62091416
TTATAGC28500.016.487723
CAAGCGA26700.016.35205815
AGCGACT26250.016.2817
TATAGCA32950.015.2716254
TCAAGCG28850.014.8769514
ATAGCAA32950.014.4855855
TAGCAAT32800.014.0442096
TCTTATA107000.013.71074837
TATACTG13350.013.4419475
TCTATAC13450.013.2044613
TCGCTAA3850.012.97402614
TATCAAC240950.012.8528753
Produced by FastQC (version 0.11.5)