FastQCFastQC Report
Fri 29 Apr 2016
ERR1307241.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307241.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7836967
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT140900.1797889413085445No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT138030.1761268102826004No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT99970.1275621040639829No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53950.029.558851
GCTTATA16250.018.2153841
GTATCAA87800.018.0996572
CGACTTA13100.017.51145219
CTTATAG16700.017.2814372
CTTATAC47550.017.27444637
GCGACTT14050.016.59074818
AAGCGAC14850.016.19528616
TTATAGC18700.015.3342243
TCTTATA76100.014.82917237
TATACCG3800.014.6052646
GTATAGA11500.014.478261
AGCGACT16850.014.27299617
TATAGCA22250.014.1348314
TATACTG12550.013.8565735
GTATTAG15800.013.1139241
CAACGCA129650.012.5283464
TTATACT16150.012.4860684
TCAACGC131850.012.4034883
ATCAACG130250.012.3996162
Produced by FastQC (version 0.11.5)