FastQCFastQC Report
Fri 29 Apr 2016
ERR1307239.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307239.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6387140
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT117630.1841669354358915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT114400.1791098989532091No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT75820.11870727743559714No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA67200.10521140917531165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55450.031.1614061
GCTTATA17300.021.0664731
GTATCAA86200.019.8091642
CTTATAC54150.019.26869837
CTTATAG18550.019.0485172
CGACTTA16550.018.33232719
GCGACTT17100.017.95906418
AAGCGAC17750.017.7183116
TTATAGC20550.017.1946473
TATAGCA22500.016.1155574
AGCGACT20650.015.14043617
ATAGCAA22450.015.0801775
TAGCAAT22850.014.73522956
TCTTATA86550.014.36395137
CAAGCGA22150.014.19864615
TTAGCGT4700.014.1702124
GTATTAG13500.013.7037031
ATCAACG126050.013.6199924
TATCAAC127000.013.5035433
CAACGCA127950.013.4177416
Produced by FastQC (version 0.11.5)