FastQCFastQC Report
Fri 29 Apr 2016
ERR1307238.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307238.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6835860
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT193650.28328549736243863No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT183550.26851047271301637No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT123810.18111839622227488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64900.031.127891
GTATCAA101650.019.8194792
CTTATAC65950.019.60803837
CTTATAG16600.017.8313262
AAGCGAC15900.017.56918116
GCTTATA17800.017.4606721
GCGACTT16000.017.3437518
TTATAGC19450.016.3598983
TCTTATA113100.016.1609237
CGACTTA16600.016.1596419
AGCGACT18200.015.55219717
TCGCTAA4900.015.47959114
TATAGCA23100.014.9761914
CTCGCTA5750.014.4782613
CAAGCGA21050.013.53444115
ATAGCAA23150.013.50545
GTATAGG7050.013.3829781
TACACAG14700.013.3401375
ATCAACG155500.012.991644
TAGCAAT24550.012.8859476
Produced by FastQC (version 0.11.5)