FastQCFastQC Report
Fri 29 Apr 2016
ERR1307237.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307237.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8573726
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT472310.5508806789486858No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT462480.5394154186872778No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT327550.382039267408359No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114440.13347755689883256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99000.031.1883831
CGACTTA18850.021.10079619
GCGACTT19850.020.13098318
CTTATAG23300.020.0085852
GCTTATA23700.019.9831221
AAGCGAC20500.019.94390116
GTATCAA161750.019.0775892
CTTATAC75200.018.54920237
AGCGACT23050.017.81778717
CAAGCGA25000.016.72415
TTATAGC27250.016.5651383
TCAAGCG26800.015.25559714
TATAGCA32950.014.7663144
TCTTATA122800.014.55293137
TAGCAAT31850.014.1726846
ATAGCAA32550.014.0384035
GACTTAA32400.013.07561720
GTATTAG17750.012.92394351
ATCAACG248400.012.8546692
TCAACGC250750.012.7194413
Produced by FastQC (version 0.11.5)