FastQCFastQC Report
Fri 29 Apr 2016
ERR1307236.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307236.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3765579
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT659691.7518952596665744No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT643691.7094051140608124No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT465621.236516349809684No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124470.33054677647182545No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA118350.3142942957776214No Hit
TATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATTT109870.2917745186065675No Hit
GGTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACAT104600.27777932689766965No Hit
GAGTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATA98320.26110194474740805No Hit
GCTTATAGCAATTTCAAGCGACTTAAATGCATATGGTGTTGTA95330.25316159878733124No Hit
GTGTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCC76350.20275766356249594No Hit
GTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATT60440.1605065250257663No Hit
CTACAACACCATATGCATTTAAGTCGCTTGAAATTGCTATAAG54890.14576775576876758No Hit
ACTTAAATGCATATGGTGTTGTAGAGTATCGACATAAAATGTG52680.13989880440697167No Hit
CTCTACAACACCATATGCATTTAAGTCGCTTGAAATTGCTATA52030.1381726422417376No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT44930.11931764012918067No Hit
CTACAACACCATATGCATTTAAGTCGCTTGAAAAAAAAAAAAA42720.11344868876738477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119450.034.2741741
GCTTATA26900.023.3141271
CTTATAG26950.022.9276432
TTATAGC28700.021.7229963
TATAGCA28450.021.4586984
GTATCAA193100.021.0867422
ATAGCAA28350.020.7513245
GCGACTT29500.019.62881518
TAGCAAT31100.019.2138256
CGACTTA29900.019.18060319
AAGCGAC31400.018.55891816
CAAGCGA31650.018.3538715
TCAAGCG31850.018.23861914
GCAATTT32050.018.1248058
AGCAATT33700.017.8961437
CAATTTC34200.016.985389
GACTTAA34150.016.73938620
AGCGACT35100.016.60256417
CTTATAC19150.016.42297737
CTCGTTA5800.016.2672422
Produced by FastQC (version 0.11.5)