FastQCFastQC Report
Fri 29 Apr 2016
ERR1307235.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307235.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7796879
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT494250.6339074904202052No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT485750.6230056924058973No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT346450.44434446141847267No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA129050.16551494514664136No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124520.15970492808725134No Hit
GAGTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATA119910.15379230587007955No Hit
GGTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACAT110370.14155664080460914No Hit
TATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATTT104440.13395103348403892No Hit
GTGTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCC93840.12035585007796068No Hit
GCTTATAGCAATTTCAAGCGACTTAAATGCATATGGTGTTGTA89650.11498190493914295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107400.032.2285841
GCGACTT24550.020.49694418
CTTATAG27950.020.3864042
GCTTATA29400.020.198981
CGACTTA25050.019.79241619
GTATCAA177750.019.4419142
AAGCGAC27150.018.8066316
AGCGACT27550.018.53357517
TTATAGC31500.017.9126993
CTTATAC43700.017.73798637
CAAGCGA30900.016.82362615
TCAAGCG32550.015.85714214
ATAGCAA35450.015.6036665
TATAGCA38400.015.51302054
GTATTAG20350.015.3636361
TAGCAAT37000.015.26
GTACTAG5900.014.4237291
TCTTATA67450.014.34469937
GACTTAA35200.014.24289820
GCAATTT36800.014.2269028
Produced by FastQC (version 0.11.5)