FastQCFastQC Report
Fri 29 Apr 2016
ERR1307233.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307233.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3759580
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT159330.42379733906447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT155840.4145143872453838No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT113520.3019486219205337No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37770.10046334962947989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39850.032.589711
GTATCAA66450.019.571862
CGACTTA5950.019.27731119
CTTATAC27250.018.94128437
AAGCGAC6650.017.52631616
TCGCTAA2850.017.52631614
CCGCCTA2700.017.1296315
GCTTATA8900.016.8370781
GCGACTT6950.016.50359718
CTTATAG8100.016.216052
AGCGACT7850.014.61146517
CTCGCTA3450.014.4782613
TCTTATA41950.014.24433937
GTATAGA8650.013.9017351
ATCAACG94500.013.7624344
TCTATAC6200.013.7258063
ATACCAT7750.013.6064516
TCAACGC96400.013.5487555
CAACGCA96500.013.5155446
GTATATA8650.013.47398951
Produced by FastQC (version 0.11.5)