FastQCFastQC Report
Fri 29 Apr 2016
ERR1307232.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307232.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6642275
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT295230.4444712090360607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT293440.4417763492177002No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT195980.29504951240350635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66650.031.5873971
GTATCAA108300.019.3711912
GCTTATA16750.018.9970151
CGACTTA13750.018.9709119
CTTATAC53100.018.84839837
CTTATAG17450.018.4469912
GCGACTT14500.017.98965618
AAGCGAC15700.017.08598716
TTATAGC20300.016.039413
AGCGACT18050.015.27146817
TCTTATA87100.014.59184837
TATAGCA23950.014.1356984
GTATTAG12250.013.8938781
ATAGCAA22600.013.8340715
CAAGCGA20550.013.77372215
TCAAGCG21050.013.71021414
TCGCTAA4100.013.53658614
TAGCAAT22850.013.1969386
ATCAACG161450.013.0514094
TCAACGC162800.012.9545455
Produced by FastQC (version 0.11.5)