FastQCFastQC Report
Fri 29 Apr 2016
ERR1307231.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307231.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9537757
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT391810.4107988911858417No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT387140.4059025617868016No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT266720.27964646195116943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108750.026.9462071
CTTATAC92450.020.23093637
GTATCAA163950.017.8511752
CGACTTA14150.015.9505319
GCTTATA19100.015.4973821
GCGACTT15400.015.49675318
CTTATAG19000.015.3842112
TCTTATA149800.014.77036137
TATACTG14950.013.8595315
AAGCGAC17150.013.8075816
GTATTAG18150.013.4545451
TTATAGC21400.013.3995323
GTATAGA14950.012.9933111
AGTATCG33100.012.7990948
ATCAACG232400.012.5854134
CCTATAC10450.012.5693783
GTACTAG8000.012.4874991
TCTATAC12750.012.4784323
TCGCTAA5950.012.43697514
TATCAAC238150.012.4136063
Produced by FastQC (version 0.11.5)