FastQCFastQC Report
Fri 29 Apr 2016
ERR1307230.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307230.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9193733
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT576310.6268509211655374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT554070.6026605297325907No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT396610.43139168822936236No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135850.14776369946788753No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA115070.12516134632145615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122450.032.5128631
CTTATAG23500.020.782982
CTTATAC106450.019.89901437
GCTTATA25250.019.8554441
GTATCAA201100.019.7419192
GCGACTT24050.018.2307718
CGACTTA23900.018.19037619
TTATAGC29850.017.7252943
AAGCGAC27300.016.0604416
AGCGACT27500.015.80909117
TCTTATA176000.015.28352437
TATAGCA36200.014.8204424
ATAGCAA33400.014.67814355
TATACAC31700.014.12302837
TCAAGCG31050.014.12077314
CAAGCGA31900.014.03448215
CGTGATA5550.014.024
TAGCAAT36050.013.9070746
TTAGACT10350.013.7632864
TAAGCAG26300.013.4353615
Produced by FastQC (version 0.11.5)