FastQCFastQC Report
Fri 29 Apr 2016
ERR1307229.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307229.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3467449
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT266170.7676248446624593No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT261880.7552526367366903No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT181300.5228627731799371No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65710.18950531067652326No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA54710.15778170061044877No Hit
GCTTATAGCAATTTCAAGCGACTTAAATGCATATGGTGTTGTA54440.15700303018155423No Hit
GAGTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATA48580.1401029979099909No Hit
GGTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACAT48360.1394685257086694No Hit
TATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATTT46340.13364291731471753No Hit
GTGTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCC38740.1117247867236115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60200.032.1137851
GCTTATA13750.024.6218171
CTTATAG14100.023.7482262
GCGACTT13650.022.09157618
AAGCGAC14600.021.1609616
AGCGACT14750.020.69491417
CGACTTA14900.020.48657619
CTTATAC32700.019.97094737
GTATCAA98050.019.6792452
ATAGCAA17100.019.4736845
TTATAGC17450.019.4011463
CAAGCGA16650.018.77777915
TATAGCA18900.018.54
TCAAGCG16900.018.39053314
GCAATTT17700.018.081928
AGCAATT19050.017.4803147
GACTTAA18550.016.55525620
GAGCGGT6100.015.7704936
TTTAGCG1557.223207E-915.516129526
TATACCG3100.015.51612956
Produced by FastQC (version 0.11.5)