FastQCFastQC Report
Fri 29 Apr 2016
ERR1307228.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307228.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6189384
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT243060.39270466980235835No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT239030.38619352103537286No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT163010.2633703127807226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64050.031.1077291
GTATCAA99850.019.9544322
CGACTTA9500.019.86315719
GCGACTT9550.019.75916118
CTTATAC43100.017.94199437
AAGCGAC10650.017.7183116
GCTTATA12650.017.5494081
CTTATAG12400.017.3064522
AGCGACT11900.016.3235317
TCGCTAA4650.016.31182714
ATCGATA6750.015.89629623
CTCGCTA5000.015.1713
TTATAGC16200.015.0740743
TCTTATA67050.014.48545837
TAATCGA7450.014.15436221
TTACTCG7350.014.09523919
CAAGCGA14500.014.03448315
GTAATCG8000.013.87500120
TACTCGC4150.013.81927720
ATCAACG145750.013.6576334
Produced by FastQC (version 0.11.5)