FastQCFastQC Report
Fri 29 Apr 2016
ERR1307227.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307227.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5927961
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT153550.2590266703846399No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT151290.25521422964827195No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT105400.17780143965184658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54300.024.9732971
CTTATAC41400.018.36594237
GTATCAA79700.016.8751562
TATATCG3150.015.2698415
CTAATCG4250.014.36470526
GTACTAG4300.014.1976761
GTCCTAA9350.013.6524061
TCTTATA63850.013.58888137
ATCGATA3950.013.58227823
GTAATCG4050.013.24691420
AATCGTC4800.013.10416728
TTAGACT6400.013.0078134
TATACTG9400.012.9893625
TAGACAG11150.012.9417055
CGACTTA4150.012.92771119
GTATAGG7450.012.9127511
GTATAGA11150.012.7757851
TAATCGA4350.012.75862121
GCTTATA7600.012.6578951
TAGACCG1050.002645805712.3333335
Produced by FastQC (version 0.11.5)