FastQCFastQC Report
Fri 29 Apr 2016
ERR1307225.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307225.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5366315
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT254180.4736583670544871No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT250590.4669684876866155No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT168550.31408890458349914No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA64300.11982151625463655No Hit
GGTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACAT55180.10282661379363679No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54900.10230484047246574No Hit
GAGTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATA54810.10213712761923219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64750.031.9142861
CGACTTA15050.021.88039819
GCGACTT15900.021.176118
GCTTATA18500.021.01
CTTATAG17950.020.6128142
GTATCAA104600.019.7026772
AAGCGAC17250.019.30434816
TTATAGC19350.018.7390173
CTTATAC41650.018.6998837
AGCGACT18300.018.49999817
CAAGCGA19300.017.63730615
TCAAGCG20550.016.29440314
ATAGCAA21800.016.2935775
TATAGCA24500.016.2346944
TAGCAAT22300.015.8452916
GCAATTT22600.014.8982318
GCGTATA1001.0945516E-414.82
TCTATAC8000.014.83
GACTTAA23100.014.7359320
CGTATCG2059.276846E-1114.43902510
Produced by FastQC (version 0.11.5)