FastQCFastQC Report
Fri 29 Apr 2016
ERR1307224.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307224.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8927001
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT424300.4752995994959561No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT410930.46032256521535064No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT287340.3218774143746595No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA103840.11632125951369335No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90390.1012546094707506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102300.031.6832851
GCTTATA26550.021.5310731
CTTATAG27800.020.363312
GCGACTT24300.020.32716218
GTATCAA159600.020.2503132
CGACTTA24350.020.1334719
CTTATAC84150.019.78609537
AAGCGAC26050.019.10364716
TTATAGC30550.018.7725053
AGCGACT28200.017.71276717
TATAGCA34950.016.9384844
TAGCAAT34400.016.4026176
CAAGCGA30850.016.25121515
TCAAGCG31400.015.9665614
ATAGCAA36400.015.3997255
TATACTG12900.014.9147295
TCTTATA142400.014.82338437
GCAATTT34900.014.6303738
GTATTAG16800.014.3154751
AGCAATT39700.013.8400517
Produced by FastQC (version 0.11.5)