FastQCFastQC Report
Fri 29 Apr 2016
ERR1307223.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307223.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9708935
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT349600.36008068856161873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT339450.34962640083593105No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT242440.24970812967642694No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA108940.11220592165876071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97950.031.3905051
GCTTATA24450.021.0347651
CTTATAC86700.020.48442837
CTTATAG25650.019.9064312
GTATCAA156900.019.5729772
CGACTTA22900.019.22707619
GCGACTT23350.019.17344718
AAGCGAC24750.018.16363716
TTATAGC30600.016.6258163
AGCGACT28000.016.38571517
ATAGCAA30800.016.0373385
TCTTATA146450.015.449337
CAAGCGA30400.014.84868415
TAGCAAT32950.014.7101686
TATAGCA36250.014.6468964
GTATTAG19400.014.3041241
TCAAGCG32050.013.96879814
TATACTG12750.013.6392165
GTATAGA15250.013.2229511
AGTATCG55350.013.1689258
Produced by FastQC (version 0.11.5)