FastQCFastQC Report
Fri 29 Apr 2016
ERR1307222.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307222.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3280563
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24500.031.0346951
GTATCAA38900.019.4035992
GTATAGG2900.019.1379321
CTTATAG7950.017.6855342
CTTATAC19400.017.3556737
CGACTTA7300.016.72602719
TTATAGC9650.016.6787553
GCGACTT7450.016.63758318
ATACCGT1352.2235326E-816.4444436
GCTTATA8450.016.4201181
AAGCGAC7800.015.891026516
TAGCCGG2100.015.8571425
TAATACG3000.015.4166674
TCTTATA30900.015.26699237
AGCGACT8300.015.15662717
GTATAGA5300.014.6603771
AATACGC2650.014.6603775
TATAGCA10750.014.4558134
GTATTAG7000.014.0071431
TAGGACG3700.014.04
Produced by FastQC (version 0.11.5)