FastQCFastQC Report
Fri 29 Apr 2016
ERR1307221.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307221.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3695511
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT89720.2427810389415699No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT86070.23290419105774546No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT58450.1581648654272711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28750.031.0800021
GTATCAA45450.019.6193622
CTTATAC22550.018.2128637
GTCGTAC700.00259395215.8571421
TGTACCG1659.767973E-1015.696975
TCTTATA35350.015.22913637
GCTTATA6050.014.9834711
CGCGGTC3600.014.90277932
TTAGACT4050.014.1604944
TAAGACG1458.9245805E-714.0344834
CGACTTA4400.013.87519
CTTATAG6150.013.8373992
CGCCGTA4300.013.76744225
ATCAACG65050.013.7079174
GCGACTT4600.013.67391318
CAACGCA66900.013.3565026
TCAACGC67050.013.3542135
GTATTAG9750.013.2820521
TTAACGG1951.0280928E-813.2820535
TATCAAC68900.013.23733
Produced by FastQC (version 0.11.5)