FastQCFastQC Report
Fri 29 Apr 2016
ERR1307219.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307219.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3575355
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT63000.17620627881706852No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT60380.16887833515832693No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41040.11478580448654749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27800.029.6798551
GTATCAA42500.019.152942
CTTATAC20100.016.93532237
CTTATAG6900.016.6231882
TCTTATA32750.015.47786337
GTATAGA6900.014.7463761
GTACTAG3400.014.6911761
ATCGATA2800.014.53571423
CTAATCG1406.004575E-714.53571426
CTAGTAC2700.014.3888893
GCGACTT5650.014.07964618
GTATTAG8250.013.903031
TCGCTAG1203.3041906E-513.87500128
ATTAGAC3200.013.8753
CGTACGA800.00630205113.87510
TAATCGA2800.013.87521
GCTTATA7950.013.7295611
ATCAACG60000.013.5358344
CGACTTA5750.013.51304319
TCAACGC60600.013.4018155
Produced by FastQC (version 0.11.5)